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KurzbeschreibungO-Ring for ApertureCartridge Rebuild KitSDS-MW Analysis Kit
Ausführliche BeschreibungFor use in the 200 and 800 um capillary cartridge UV aperture.This item contains contains the capillary cutting length template, cleaving stone, rebuild instructions, O−rings, installation tool, and tweezers. This item works with all of SCIEX's capillary offerings (sold separately). The cartridge body is also sold separately.The SDS-Gel MW Analysis Kit is designed for use on the PA 800 Plus and is used for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID, Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • MW Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL
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